Sequence of a full length cDNA coding for human protein HC (α1microglobulin)

cDNA sequence coding for a translationally controlled human tumor protein.

The sequence presented was obtained by screening a cDNA library constructed in lambda gtlO from human mammary carcinoma with probes derived from cDNA of the translationally controlled, growth-related mouse tumor protein p23 (1,2). It has 86% similarity to the mouse cDNA sequence and encodes a protein with 96% similarity to the mouse tumor protein. The 3-primed noncoding region of the appropriat...

Molecular cloning and nucleotide sequence of full-length of cDNA coding for porcine gastrin.

We have cloned in EScherichia coli a cDNA copy of mRNA coding for the porcine antral mucosal hormone preprogastrin. Full-length double-stranded cDNA was synthesized and inserted into the Pst I endonuclease site in plasmid pBR322 by using a homopolymeric extension technique. A partial cDNA clone was used as a probe to identify a complete cDNA clone in a cDNA library by colony hybridization. Four...

Isolation and characterization of a full-length cDNA coding for an adipose differentiation-related protein.

We have previously isolated from a 1246 adipocyte cDNA library a cDNA clone called 154, corresponding to a mRNA that increases abundantly at a very early time during the differentiation of 1246 adipocytes and in adipocyte precursors in primary culture. We show here that the mRNA encoded by this cDNA is expressed abundantly and preferentially in mouse fat pads. A full-length cDNA for clone 154 w...

An Integrated Database System for Full-Length Human cDNA in NEDO Full-Length Human cDNA Sequencing Project

Isolation and characterization of full-length cDNA clones coding for cholinesterase from fetal human tissues.

To study the primary structure and regulation of human cholinesterases, oligodeoxynucleotide probes were prepared according to a consensus peptide sequence present in the active site of both human serum pseudocholinesterase (BtChoEase; EC 3.1.1.8) and Torpedo electric organ "true" acetylcholinesterase (AcChoEase; EC 3.1.1.7). Using these probes, we isolated several cDNA clones from lambda gt10 ...